Microscopy:
Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Software:
Article Title: Establishment of Wnt ligand-receptor organization and cell polarity in the C. elegans embryo
Article Snippet: .. Fluorescence imaging was performed on two spinning disk confocal microscopes equipped with CSU-X1 spinning disk unit (Yokogawa) mounted on Nikon Ti eclipse stands: (1) 100x/1.49NA Nikon objective, EMCCD Andor iXon3 DU897, MicroManager software , (2) 100x/1.4 Nikon objective, EMCCD Photometrics Evolve (Teledyne), Metamorph software (Molecular devices). ..
Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Fluorescence:Article Title: Establishment of Wnt ligand-receptor organization and cell polarity in the C. elegans embryo
Article Snippet: .. Fluorescence imaging was performed on two spinning disk confocal microscopes equipped with CSU-X1 spinning disk unit (Yokogawa) mounted on Nikon Ti eclipse stands: (1) 100x/1.49NA Nikon objective, EMCCD Andor iXon3 DU897, MicroManager software , (2) 100x/1.4 Nikon objective, EMCCD Photometrics Evolve (Teledyne), Metamorph software (Molecular devices). ..
Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Imaging:Article Title: Establishment of Wnt ligand-receptor organization and cell polarity in the C. elegans embryo
Article Snippet: .. Fluorescence imaging was performed on two spinning disk confocal microscopes equipped with CSU-X1 spinning disk unit (Yokogawa) mounted on Nikon Ti eclipse stands: (1) 100x/1.49NA Nikon objective, EMCCD Andor iXon3 DU897, MicroManager software , (2) 100x/1.4 Nikon objective, EMCCD Photometrics Evolve (Teledyne), Metamorph software (Molecular devices). ..
Confocal Microscopy:Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Control:Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Transmission Electron Microscopy:Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Raman Spectroscopy:Article Title: The interface of condensates of the hnRNPA1 low-complexity domain promotes formation of amyloid fibrils.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Widefield fluorescence / brightfield microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Plate reader software for Thioflavin T profiles: Reader Control and MARS softwares (BMG Labtech) Raman spectra acquisition: LabVIEW 2020 software, Labspec 6.5, Multiwell, EasyNav (National Instruments) Confocal microscopy: Leica LAS X SP8 software (version 1.0) TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplet size distributions and numbers: in-house written program in Matlab (version R2020a) Image analysis: Fiji/ImageJ (version 2.3.0) Raman spectroscopy analysis: Python (version 3.9.5), Wavemetrics IgorPro 9 software (version 9.01) AFM: Nanoscope 8.1 software (Bruker) All data were plotted using Matlab (version R2020a) Steric zipper prediction: ZipperDB, https://services.mbi.ucla.edu/zipperdb/ LARKS prediction: LARKSdb, https://srv.mbi.ucla.edu/LARKSdb/index.py For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Spectroscopy:Article Title: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Bright field microscopy: MicroManager software (version 2.0 gamma) Re-scan confocal microscopy: NIS Elements 5.20 Advanced research software (Nikon) Confocal microscopy: Leica LAS X SP8 software (version 1.0) Plate reader for ThioflavinT assays: Reader Control and MARS software (BMG Labtech) Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 TEM Morgagni: iTEM 5.2 and MorgagniUI Version 3 Data analysis Droplets diameter, number, surface/volume ratio : in-house written programs in Python (version 3.9.5) Image Analysis and FDLD calculation: Fiji 2.9.0 All data were plotted using R studio 4.1.2 Protein disorder prediction: IU pred https://iupred2a.elte.hu RNA secondary structure prediction: RNA fold WebServer http://rna.tbi.univie.ac.at//cgi-bin/RNAWebSuite/RNAfold.cgi? .. PAGE=3&ID=XS3fKUfcoq Fluoresce Correlation Spectroscopy: Leica LAS X SP8 Version 1.0 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.
Incubation:
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